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Calcitriol concentration following 24 of treating MAP-infected macrophages with 50 ng/mL of calcifediol (A) , and verification of successful cathelicidin knockdown by <t>CAMP-siRNA</t> (B) . Values were determined by Calcitriol ELISA Kit (MyBioSource, San Diego, CA) and the Human Cathelicidin Antimicrobial Peptide ELISA Kit (MyBioSource, San Diego, CA). *Indicates P-value of less than 0.05.
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Calcitriol concentration following 24 of treating MAP-infected macrophages with 50 ng/mL of calcifediol (A) , and verification of successful cathelicidin knockdown by <t>CAMP-siRNA</t> (B) . Values were determined by Calcitriol ELISA Kit (MyBioSource, San Diego, CA) and the Human Cathelicidin Antimicrobial Peptide ELISA Kit (MyBioSource, San Diego, CA). *Indicates P-value of less than 0.05.
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Calcitriol concentration following 24 of treating MAP-infected macrophages with 50 ng/mL of calcifediol (A) , and verification of successful cathelicidin knockdown by <t>CAMP-siRNA</t> (B) . Values were determined by Calcitriol ELISA Kit (MyBioSource, San Diego, CA) and the Human Cathelicidin Antimicrobial Peptide ELISA Kit (MyBioSource, San Diego, CA). *Indicates P-value of less than 0.05.
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Calcitriol concentration following 24 of treating MAP-infected macrophages with 50 ng/mL of calcifediol (A) , and verification of successful cathelicidin knockdown by CAMP-siRNA (B) . Values were determined by Calcitriol ELISA Kit (MyBioSource, San Diego, CA) and the Human Cathelicidin Antimicrobial Peptide ELISA Kit (MyBioSource, San Diego, CA). *Indicates P-value of less than 0.05.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Cathelicidin Mediates an Anti-Inflammatory Role of Active Vitamin D (Calcitriol) During M. paratuberculosis Infection

doi: 10.3389/fcimb.2022.875772

Figure Lengend Snippet: Calcitriol concentration following 24 of treating MAP-infected macrophages with 50 ng/mL of calcifediol (A) , and verification of successful cathelicidin knockdown by CAMP-siRNA (B) . Values were determined by Calcitriol ELISA Kit (MyBioSource, San Diego, CA) and the Human Cathelicidin Antimicrobial Peptide ELISA Kit (MyBioSource, San Diego, CA). *Indicates P-value of less than 0.05.

Article Snippet: 5 nmol of SilencerTM Pre-Designed siRNA (siRNA ID: 14402, ThermoFisher, Waltham, MA) specific to CAMP were diluted first in 50 uL nuclease-free H 2 O.

Techniques: Concentration Assay, Infection, Knockdown, Enzyme-linked Immunosorbent Assay, Peptide ELISA

Effects of CAMP-siRNA transfection on the expression of TNF-α (A) , IL-1β (B) , and IL-10 (C) in MAP-infected macrophages. *Indicates P-value of less than 0.05.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Cathelicidin Mediates an Anti-Inflammatory Role of Active Vitamin D (Calcitriol) During M. paratuberculosis Infection

doi: 10.3389/fcimb.2022.875772

Figure Lengend Snippet: Effects of CAMP-siRNA transfection on the expression of TNF-α (A) , IL-1β (B) , and IL-10 (C) in MAP-infected macrophages. *Indicates P-value of less than 0.05.

Article Snippet: 5 nmol of SilencerTM Pre-Designed siRNA (siRNA ID: 14402, ThermoFisher, Waltham, MA) specific to CAMP were diluted first in 50 uL nuclease-free H 2 O.

Techniques: Transfection, Expressing, Infection

Effect of CAMP-siRNA transfection on cytokine production in MAP-infected macrophages.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Cathelicidin Mediates an Anti-Inflammatory Role of Active Vitamin D (Calcitriol) During M. paratuberculosis Infection

doi: 10.3389/fcimb.2022.875772

Figure Lengend Snippet: Effect of CAMP-siRNA transfection on cytokine production in MAP-infected macrophages.

Article Snippet: 5 nmol of SilencerTM Pre-Designed siRNA (siRNA ID: 14402, ThermoFisher, Waltham, MA) specific to CAMP were diluted first in 50 uL nuclease-free H 2 O.

Techniques: Transfection, Control, Infection